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Fig. 8. Wounding and adhesion enhance binding of CEACAM1 to FLNa thereby disturbing the interaction of FLNa with the small GTPase RalA. (A) A7-CC1 cells were left untreated, wounded with a multichannel pipette, allowed to re-adhere to laminin-1, or kept in suspension for 1 hour prior to lysis. Lysates were immunoprecipitated with anti-CEACAM1 mAb 4/3/17. Blots of precipitations and of lysates were developed for co-precipitated FLNa with mAb FLMN01 and quantified. Mean amounts of FLNa co-precipitated by CEACAM1 are shown in the diagram with their standard deviations. (n=3) (B,C) Ral activation assay. A7 and A7-CC1 cells were treated as described above and precipitated with Sepharose-bound Ral-GTP binding domain. Blots of precipitations and of lysates were developed for RalA (B) and for FLNa (C) and quantified. Mean amounts of Ral-GTP and of co-precipitated FLNa are shown in the diagrams with their standard deviations (n=3).
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