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Fig. 2. btn1{Delta} cells have larger vacuoles. (A) Wild-type cells (top panels) and btn1{Delta} cells (lower panels) labelled with FM4-64 and observed by fluorescence microscopy during exponential growth (left panels) or hypotonic shock (right panels). Bar, 5 µm. (B) Vacuole size distribution before (upper panel) and after (lower panel) hypotonic shock in wild-type cells (black bars) and btn1{Delta} cells (white bars). Vacuole size is increased in btn1{Delta} cells before and after fusion in response to hypotonic shock.





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