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Fig. 5. btn1 and vma1 show conditional synthetic lethality. (A) btn1 ${Delta}$ vma1 ${Delta}$ cells were viable at 25°C but Btn1 protein was essential for survival at 30°C. (B) DAPI and calcofluor staining of cells deleted for vma1 and btn1vma1 at 36°C. btn1 ${Delta}$ vma1 ${Delta}$ cells were unable to initiate septum formation and many were elongated. Bar, 10 µm.

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