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Fig. 7. Spatial and global quantification of P-MLC levels during keratinocyte polarization. (A) P-MLC levels were quantified at thin bundles and in the cell body from confocal images obtained after 30 minutes of induction of cell-cell contacts. Following junction formation (Std. Ca2+), an increase in fluorescence intensity is seen both at thin bundles (2.7 fold) and in the cell body (1.4 fold). Values are from two independent experiments, using at least 50 different cells per condition. (B) Western blots showing the overall increase in P-MLC levels in keratinocytes. Quantification reveals that a threefold increase in phosphorylation levels is seen after 15 minutes of new junction formation (P<0.001). Values are the mean of four independent experiments.





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