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Fig. 4. Localization of rgf1+ and rgf3+. (A) Schematic representation of Rgf1 and Rgf3 protein domain structures. Both proteins contain RhoGEF, pleckstrin homology (PH) and citron homology (CNH) domains. In addition, Rgf1 contains a DEP (dishevelled, Egl-10 and pleckstrin) domain. The asterisk denotes the F867S mutation found within rgf3 in the lad1-1 strain. (B-D) Localization of Rgf1p-GFP in live cells (B,C) and Rgf3p-GFP in fixed cells (D) (KGY4404 and KGY4407, respectively). (C) The ring of Rgf1p-GFP viewed from the side and en face. (E) Model of Rgf1p and Rgf3p localization during cell division.





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