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Fig. 5. Cilium structure analysis of nph-1;nph-4 double mutants. (A) Cilium structure was analyzed by evaluating the ability of nph-1, nph-4 and double nph-1;nph-4 mutants to absorb DiI (dye-filling assay). There were no overt differences detected between any of the mutant strains when compared to that of wild-type controls. (B) Cilium structure was further analyzed in nph-1;nph-4 double mutants using the CHE-13::YFP fusion protein. Cilium structure was indistinguishable from that of wild-type controls and CHE-13::YFP was properly localized throughout the cilium. (C) To evaluate possible effects of the nph mutants on cilium structure, the length of cilia was determined in nph-1;nph-4 double mutants expressing CHE-13::YFP. There were no statistically significant differences detected in the cilia of wild type and double mutant lines. Identical results were obtained with nph-4 single mutants (data not shown).





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