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Table 2. Gene-dosage-dependent suppressors of PI 3-phosphatase-deficient mutant inviability

ORF Gene name SYD Rescues ymr1^ts^* PtdIns(3)P level (% of ymr 1^ts) Cellular function

Complementing lipid phosphatases

YJR110W YMR1 3,25 ++++ 34.5 PI 3-phosphatase

YNL106C SJL2 28 ++++ 44.0 Polyphosphoinositol phosphatase

SJL2-T755 9 ++++ ND

YOR109W SJL3-T893 2,4 ++++ 28.5 Polyphosphoinositol phosphatase

SJL3-T571 21 ++ 70.9

Cell-integrity MAPK pathway regulation

YBL105C PKC1-T615 5,6,22,27 ++++ 129.3 Protein kinase C; isolates lack the C-terminal kinase domain

PKC1-T794 7

YDR389W SAC7 14 ++ ND GAP for Rho1p GTPase

YNL053W MSG5 34 ++ 102.1 Protein phosphatase that regulates MAP kinases

Membrane homeostasis

YHR001W OSH7 32 ++ 125.4 Oxysterol-binding protein

Vesicle trafficking

YFL038C YPT1 20 ++++ 111.7 RAB GTPase, ER-Golgi trafficking

YDL077C VAM6 35 ++ 95.5 Ypt7p activator, Endosome-vacuole fusion

^* The number of tenfold serial dilution spots (4 total) that contained significant growth compared with ymr1^ts sjl2 ${Delta}$ sjl3 ${Delta}$ control cells rescued with wild-type YMR1 after 3 days at 35°C is indicated by +.

Values are representative of three experiments conducted at 35°C where ymr1^ts mutant cells had PtdIns(3)P levels of 4.3±0.32% of the total ³H phosphoinositides detected, therefore a % change of 7.5 or less may not be significant.

Cellular function as described by the Saccharyomyces Genome Database.

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