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Fig. 7. Localisation of PfGRASP depends on a functional N-terminal myristoylation motif. Fluorescence microscopy was performed on live parasites. (a) In parasites expressing PfGRASP-GFP the protein is restricted to two tightly defined compartments (green). (b) Merge with bright-field image. (c) Mutation of the putative N-terminal myristoylation site (from glycine to alanine) abolishes targeting of PfGRASP-GFP and results in a cytoplasmic distribution of the fusion protein. (d) Merge with bright-field image. Bar, 2 µm.





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