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Fig. 8. Effect of Brefeldin A on the distribution of PfGRASP. (a) Parasites were incubated with BFA for 24 hours. Localisation of PfGRASP-GFP in live parasites is focused to one compartment within the parasite (green). (b) Merge with bright-field image. (c) As a control, cultures were incubated with ethanol to ensure normal growth and morphology. The image displays a mature parasite (>32 hours) with multiple fluorescing foci (green). (d) Merge with bright-field image. Bar, 2 µm.





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