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Fig. 5. Rate of recovery of CysLT₁ functionality after homologous or heterologous desensitization in dU937 cells and in the D5 clone, and effect of PTX or YM-254890 on extracellular nucleotide-induced heterologous desensitization. (A) Time course of recovery of CysLT₁ functionality (10 nM LTD₄, 2nd challenge) following homologous and heterologous desensitization in dU937 cells. Values shown represent means of [Ca²⁺]_i stimulation over basal (s/b) ± s.e.m. of five independent experiments. (B) Time course of recovery of CysLT₁ functionality (10 nM LTD₄, 2nd challenge) following homologous desensitization in control dU937 cells and D5 clone. Values shown represent means of [Ca²⁺]_i stimulation over basal (s/b) ± s.e.m. of at least three independent experiments. (C) Effect of 300 ng/ml PTX pretreatment (overnight) on the [Ca²⁺]_i transient induced by 10 nM LTD₄ (2nd challenge) after an initial challenge with 10 µM ATP or 100 µM UDP. Values shown represent the percentage response ± s.e.m. of three independent experiments. (D) Effect of 60 nM YM-254890 pretreatment (5 minutes) on the [Ca²⁺]_i transient induced by 10 nM LTD₄ (2nd challenge) after an initial challenge with 10 µM ATP or 100 µM UDP. Values shown represent the percentage response ± s.e.m. of three independent experiments.

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