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Fig. 6. Equilibrium binding and acid wash of [3H]LTD4 in intact dU937 cells. (A) Percentage of total [3H]LTD4 binding and displacement by unlabeled LTD4, ATP or UDP. (B) Effect of a 30 minute pretreatment with 0.1 µM LTD4, 100 µM ATP or 1 mM UDP on surface-specific [3H]LTD4 binding. (C) Acid wash: effect of the incubation at 0°C or 37°C (30 minutes) on the distribution of total [3H]LTD4 radioactivity (Tot); P, pellet; S, supernatant. Values shown represent means±s.e.m. of at least two independent experiments.





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