QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 8. Effect of SV2A and SV2C silencing on cytosolic and vesicular [Ca²⁺]. (A) INS-1E cells were transiently co-transfected with GFP and an empty vector (Control), or with either the SV2A siRNA-3 (SV2A) or the SV2C siRNA-2 (SV2C). Three days later, the cells were infected with AdCAcAq, further cultured for 24 hours and separated by FACS for enrichment of transfected cells. (i) After cytosolic aequorin reconstitution, the cells were perifused with 2.5 mM glucose and stimulated with 30 mM KCl as indicated. (ii) Peak of [Ca²⁺]_c. Data show the mean±s.e. of four independent traces. (B) INS-1E cells were co-transfected as above. The day after, the cells were infected with AdCAVAMP.Aq, further cultured for 72 hours and separated by FACS. (i) The cells were perifused as detailed in Fig. 5B, panel b. (ii) Peak of [Ca²⁺]_SG. Values represent the mean±s.e. of four independent experiments.

Return to article