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Fig. 2. Slowed migration of AQP4-deficient astroglia in vitro. (A) Representative images (left) of Transwell migration assay using 10% FBS as chemoattractant, showing wild-type astroglia (+/+) and AQP4-null astroglia (–/–) before (pre) and after (post) scraping the non-migrated cells. Cells were stained with Coomassie Blue. Data summary (right) of migration experiments towards 10% FBS (15 wild-type vs. 13 AQP4-null Transwells; *P<0.001) or 1% FBS (1 wild-type vs. 1 AQP4-null Transwell). (B) Phase-contrast micrographs (left) showing astroglial cultures immediately after (0 h) and 48 hours after scratch. Dashed yellow lines indicate wound edges. Summary (right) of astroglial migration data at 24 and 48 hours after scratch (24 wild-type vs. 24 AQP4-null astroglial cultures per time point). Data are mean±s.e.m. Differences are significant at 24 hours (*P<0.01) and 48 hours (*P<0.001). Bar, 100 µm (A); 400 µm (B).

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