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Fig. 5. Astroglial cell migration after stab injury in vivo. (A) Reactive astroglia (arrowheads) stain strongly for GFAP (brown) and have multiple processes. (B-C) Stab injury produces a front of reactive astroglia (blue line) that migrates towards (arrows) the margin of injured brain (red line). Representative data for wild-type mice at 3 days (B) and 7 days (C) after stab injury. (D-E) Astroglial front (blue line) and margin of stab injury (red line) in wild-type (D) and AQP4-null (E) mouse at 3 days. Arrows indicate direction of migration of the reactive astroglial front. (F) Average distance of reactive astroglial front from the edge of the stab wound at 3 days after stab injury. Data are mean±s.e.m. *P<0.001. Bar, 50 µm (A); 100 µm (B-C); 400 µm (D-E).





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