QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. APC in neurite tips is along microtubules and changes during neuronal polarization. (A) An axonal growth cone of a hippocampal neuron immunostained for tubulin and APC. Projection of deconvolved planes is shown. (Note that deconvolution resolves APC enrichment into puncta.) (B) Immunostaining for the axon marker dephospho-tau (Tau) and APC show APC enrichment at several neurite tips in an unpolarized neuron and only at the tip of the axon (arrowhead), which is marked by dephospho-tau staining (arrow), in a polarized neuron. Magnified images below also show phase-contrast images of neurite tips N3 and N4 from the unpolarized neuron and the axon tip from the polarized neuron. A, axon; D, dendrite; N, neurite. (C) Line scans quantifying fluorescence intensity of APC (red) and dephospho-tau (Tau, green) along corresponding neurites from B. (D) From line scans of polarized neurons (including that in C), we calculated the ratio of maximum APC intensity at the neurite tip to that of equivalent length of the neurite shaft. Shown is the mean±s.e.m. of the axon and the longest dendrite of 13 neurons. ***P<0.001 compared to APC enrichment in axons by Student's t-test. Bar, 2 µm (A); 20 µm (whole-cell images in B); 2.5 µm (magnified images in B).

Return to article