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Fig. 4. Stabilized ß-catenin accumulates with APC clusters independently of the transcriptional activation domain. Transfected PC12 cells were treated with NGF for 3-4 days to induce extensions. (A,B) Deconvolved images of immunostaining for APC (first column) and KT3 epitope tag of *ß-catenin or *ß-cat{Delta}C-term (middle column). (C) Immunostaining for tubulin (first column) and myc tag of ß-cat-eng (middle column). *ß-catenin, full-length stabilized ß-catenin; *ß-cat{Delta}C-term, stabilized ß-catenin lacking the C-terminal transcriptional transactivation domain; ß-cat-eng, ß-catenin lacking the transactivation domain and fused to a transcriptional repression domain from Engrailed. Arrows indicate neurite tips magnified in inset and arrowheads indicate all other neurite tips. Compared to endogenous ß-catenin (Fig. 3B), stabilized ß-catenin is enriched in APC clusters at neurite tips (A,B). Bar, 10 µm.





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