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Fig. 2. Effects of elt-1 RNAi applied during embryogenesis. (A) Homozygous arrested elt-1(zu180) embryo expressing the ijIS12 dpy-7::GFP transgene. (B) RNAi arrested elt-1 embryo expressing the dpy-7::GFP transgene. (C) RNAi of elt-1 in L1 larvae with Lpy-Dpy phenotype. (D) JR1000 L1 larva showing the wild-type expression pattern of the SCM::GFP and ajm-1::GFP reporter genes. (E) RNAi of elt-1 in Lpy-Dpy JR1000 larva showing loss of SCM::GFP and ajm-1::GFP expression and disorganisation of the remaining seam cells. (F) Normal dpy-7::GFP L1 expression pattern from the ijIS12 transgene. (G) RNAi of elt-1 Lpy-Dpy L1 larvae expressing dpy-7::GFP from the ijIS12 transgene showing loss of expression in positions that should be occupied by seam cells (arrows). (H) Wild-type JG5 L1 larvae showing normal elt-3::GFP expression in the dorsal/ventral hypodermis (but not the lateral seam cells) and cells immediately anterior and posterior to the gut (virL, virR, rect D, rect VL and rect VR) (Gilleard et al., 1999). (I) RNAi of elt-1 Lpy-Dpy L1 larva showing loss of elt-3::GFP expression from the vpIS1 transgene in all the hypodermal cells of the head and dorsal/ventral hypodermis. A C. elegans embryo is approximately 50 µm in length. A newly hatched L1 larva is approximately 240 µm in length.

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