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Fig. 4. Seam-cell loss induced by postembryonic elt-1 RNAi. (A) Mean number of SCM::GFP expressing cells in different stages following elt-1 RNAi (unilateral counts, n=10; error bars indicate s.d.). N2 indicates the JR667 strain, wIs51 SCM::GFP in N2 background, and NL indicates the JG147 strain, wIs51 SCM::GFP marker in rrf-3(pk1426) RNAi-hypersensitive mutant background. (B,C) Negative-control JR667 and JG147 L4 larvae, respectively. (D,E) RNAi of elt-1 in JR667 and JG147 L4 larvae respectively. (F) RNAi of elt-1 in JR1000 L4 larva showing loss of expression of both the SCM::GFP and ajm-1::GFP markers. (G) RNAi of elt-1 in JR667 L4 larva with dispersed SCM::GFP expression in a degenerate seam cell (arrow). (H) Mean number (±s.d.) of SCM::GFP expressing cells (unilateral), number of SET cells and number of bursal rays of the male tail after elt-1 RNAi. (I) SEM of a normal male tail. (J) SEM of male tail following elt-1 RNAi. An adult C. elegans hermaphrodite is approximately 1 mm in length.





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