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Fig. 2. A novel histone H2B variant colocalizes with H2AZ. (A) The sequence of H2BV aligned with T. brucei H2B (Tb10.406.0330). Amino acids conserved between H2BV and H2B are highlighted. (B) A bloodstream-form cell line expressing TY1-tagged H2BV was examined using antibodies that recognize TY1 (green) and H2AZ (red). DNA (blue) was detected with DAPI. The H2BVand H2AZ signals are completely coincident (yellow) at all stages of the cell cycle. Top, two interphase cells (1n 1k); bottom, a late mitotic cell (2n 2k). Bar, 2 µm.





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