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Fig. 3. H2AZ and H2BV show similar genomic distributions. (A) Chromatin immunoprecipitations were carried out on cell lines expressing TY1-H2A, TY1-H2AZ, TY1-H2BV, or parental `wild-type' cells. For each cell line, chromatin was immunoprecipitated using nonspecific ( ${alpha}$ FLAG) or specific ( ${alpha}$ TY1) antibodies. Input chromatin (10% of total) and immunoprecipitated material were analyzed by slot blot using radiolabeled probes to a variety of sequences: transcriptionally active genes [ ${alpha}$ -tubulin ( ${alpha}$ TUB), ß-tubulin (ßTUB), histone H3 (HHT), 5SDNA and VSG221]; silenced genes [procyclin EP1 (EP1), VSG224 and VSGVO2]; a retrotransposon-like element (INGI); and repetitive, noncoding sequences [rDNA spacer region (rDNA spacer), expression-site promoter (ES pro), telomeric repeat (TEL), mini-chromosome 177 bp repeat (MC177) and 50 bp repeat (50 bp)]. (B) Quantification of the data in A. Using a phosphorimager, each signal was quantified and the amount of material immunoprecipitated relative to the input was calculated. Each ChIP was performed 2-4 times and the values averaged. Error bars are indicated.

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