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Fig. 5. H2AZ and H2BV co-immunoprecipitate. Mononucleosomes were prepared from cell lines expressing TY1-H2BV, FLAG-H2B, TY1-H2A or TY1-H3V, and were subjected to immunoprecipitations using antibodies to TY1 or FLAG. Immunoprecipitated material was then examined by western blotting for the presence of the TY1- or FLAG-tagged histone along with H2AZ and H3. For each experiment, a lane corresponding to whole-cell extract (wce), input material (input) and immunoprecipitated material (IP) is shown. (A) Both H2AZ and histone H3 co-immunoprecipitate with TY1-H2BV. (B) Histone H3, but not H2AZ, co-immunoprecipitates with FLAG-H2B. (C) Histone H3, but not H2AZ, co-immunoprecipitates with TY-H2A. (D) Neither H2AZ nor histone H3 co-immunoprecipitate with TY1-H3V.





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