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Fig. 7. Abnormal actin organization in TLS-null dendrites. Mouse primary hippocampal neurons prepared from either wild-type or TLS-null mice were fixed at 21 DIV, and then stained with Oregon Green phalloidin to visualize F-actin. (A) Actin organization in wild-type (A1) and TLS-null dendrites (B1). Wild-type dendrites normally have mushroom-shaped spines (arrowheads in A2). By contrast, numbers TLS-null dendrites did not have distinct heads to the spines (B2, arrows) or they produced long, thin, filopodia-like protrusions (B3, arrows). Bar, 20 µm in B1, 5 µm in B3.





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