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Fig. 2. Mitotic behaviour of microtubules in rqh1 ${Delta}$ cells. (A) Cells expressing GFP– ${alpha}$ 2-tubulin were synchronously released from the G2 block imposed by a temperature-sensitive cdc25-22 mutation. Samples were taken every 10 minutes and percentages of cells with different lengths of mitotic spindles in the indicated strains were determined. Representative examples of cells with metaphase (I) and anaphase (II) spindles in each strain are shown: Spindle (GFP-Atb2) in green and DNA (DAPI) in blue. Bar, 10 µm. (B) Visualisation of mitotic spindle behaviours in living rqh1 ${Delta}$ cells. Individual wild-type and rqh1 ${Delta}$ cells expressing GFP– ${alpha}$ 2-tubulin were observed over a period of 20 minutes or 36 minutes as in (C), with images collected every 4 minutes.

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