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Fig. 3. rqh1 ${Delta}$ cells are defective in nucleolar segregation. (A) Merged images of fluorescence micrographs showing Gar2-GFP and DNA (Hoechst 33342) localisation in living wild-type and rqh1 ${Delta}$ cells. Bar, 10 µm. (B) Visualisation of lagging Gar2-GFP signal in rqh1 ${Delta}$ cells. Individual cells of indicated strains expressing Gar2-GFP were observed as in A, over a period of 20 minutes, with images collected every 4 minutes. (C) Percentages of cells displaying aberrant nucleolar structures in each strain are shown (n=200).

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