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Fig. 5. The spindle checkpoint is activated in rqh1 ${Delta}$ mutants. (A) mad2 ${Delta}$ and mad2 ${Delta}$ rqh1 ${Delta}$ cells expressing GFP– ${alpha}$ 2-tubulin were synchronously released from the G2 block imposed by a temperature-sensitive cdc25-22 mutation. Samples were taken every 10 minutes and percentages of cells with mitotic spindles in the indicated strains were determined. (B) Merged images of fluorescence micrographs showing Mad2-GFP and DNA (Hoechst 33342) localisation in living wild-type and rqh1 ${Delta}$ cells. (C) Visualisation of Cut2-GFP signals in methanol/acetone-fixed, DAPI-stained cells. The frequencies of cells with GFP signals in B and C are shown. Bar, 10 µm.

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