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Fig. 7. Temporal analysis of gene expression by T. gondii infection. WT, IKK^–/–, IKKß^–/– and IKK^–/–ß^–/– MEFs were infected with T. gondii at an m.o.i. of 5:1 and total RNA was isolated at the time points indicated. Expression of IL-6 (A), GRO1 (B) and IAP-2 (C) was determined by real-time RT-PCR analysis as described in the Materials and Methods. Infection of WT cells resulted in fluctuating patterns of IL-6 (A) and GRO1 (B) activation; these fell into two major phases determined by an early host IKK response (1-3 hours) and an intermediate-to-late TgIKK response (9-24 hours). Contrary to this, a marked decrease of IL-6 and GRO1 expression was observed in IKK-knockout cells. A disruption of the IKK complex did not abrogate the expression of IAP2 to the same extent as observed with IL-6 and GRO1 (C), thus the temporal analysis of IAP2 lacked the characteristic biphasic response observed with early response genes. *, P<0.05 when comparing the levels of expression at the 9 hours time point of WT cells to all other time points in IKK^–/–, IKKß^–/– and IKK^–/–ß^–/– cells.

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