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Fig. 6. Overexpressed LEM2 accumulates in peripheral patches. (A) HeLa or C2C12 cells expressing high levels of LEM2-V5 were processed for indirect immunofluorescence microscopy using anti-V5 antibody (red) and DNA was stained with Hoechst dye (blue). Bars, 10 µm. (B) Confocal images of cells in xy and xz optical sections. White line indicates location of xz section. Bars, 2 µm. (C) Transmission electron microscopic images of thin sections of embedded wild-type HeLa cells (left) or hLEM2-overexpressing HeLa cells (right). Bars, 5 µm.





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