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Fig. 7. A subset of NE proteins is recruited to LEM2 patches. HeLa cells constitutively expressing high levels of V5-tagged hLEM2 were processed for immunofluorescence microscopy using anti-V5 antibody (green) and antibodies to different NE proteins (red). Bottom row shows images of HeLa cells stably expressing V5-tagged MAN1 (red) and co-transfected with human LEM2-GFP (green)-encoding plasmid. Images on the right show untransfected HeLa cells stained for the NE proteins indicated (control; red) or cells expressing V5-tagged MAN1 alone (bottom row). Arrowheads in left image (row 4) depicts lamin A patches. Bars, 10 µm.





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