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Fig. 8. LEM2-induced tubules are stable connections, persisting throughout several cell cycles. HeLa cells stably overexpressing V5-tagged hLEM2 were processed for immunofluorescence microscopy using antibodies to indicated proteins. Images depict LEM2 stained with anti-V5 (green) and actin (A, red) or phospho-histone H3 (C, red) or BAF (F, red), and DNA (A-E, blue). Arrows in (C) indicate cells shown at higher magnification in (E). Arrows in (E) depict LEM2 patches from which tubular structures emerge. Single colour images corresponding to the merged images in A and B are shown. Bars, 10 µm.





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