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Fig. 10. PKL-paxillin interaction is necessary for PTP-PEST to regulate cell spreading. PTP-PEST–/– cells were transiently transfected with GFP with or without PTP-PEST, together with paxillin {Delta}LD4, PKL or the PKL {Delta}PBS2 mutant, plated on fibronectin-coated coverslips for 60 minutes and processed for immunofluorescence. Results represent the percentage of round GFP-positive cells. Results show mean±s.d. A minimum of 100 cells were counted in three independent experiments. **P<0.001, *P<0.05.





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