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Fig. 5. Paxillin is required for PTP-PEST to inhibit cell protrusiveness. Paxillin–/– cells transfected with GFP and the PTP-PEST and paxillin constructs as indicated were plated on 10 µg/ml fibronectin-coated coverslips for 180 minutes; thereafter, time-lapse images were taken every 10 minutes. Changes in cell protrusion area of a minimum of 10 transfected cells were quantified at 10-minute intervals for 1 hour as described in Materials and Methods. **P<0.001, *P<0.05.





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