spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. PTP-PEST regulates Rac1 activation through an interaction with paxillin. (A) PTP-PEST–/– cells or (B) paxillin–/– cells were co-transfected with Myc-tagged wild-type Rac1 together with GFP, PTP-PEST, PTP-PEST and paxillin, or PTP-PEST and paxillin C523S, and either held in suspension or plated on fibronectin-coated culture dishes for 60 minutes. The level of activated Rac was determined using a GST-PAK-PBD pull-down assay as described in Materials and Methods. Total and active Rac levels were visualized by western blotting and quantitative densitometry was performed. (C,D) Results show the mean±s.d. of three independent experiments. PTP-PEST inhibited adhesion-induced Rac activation only in the presence of paxillin. This effect was reversed by introduction of the paxillin C523S mutant defective in PTP-PEST binding. **P<0.001.





Right arrow Return to article