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Fig. 8. Rac1 rescues paxillin-dependent PTP-PEST suppression of cell spreading. (A) Paxillin–/– cells were cotransfected with constitutively active (V12 Rac) or dominant negative (N17 Rac) Rac together with GFP, and with PTP-PEST and paxillin, PTP-PEST and paxillin {Delta}LD4, PTP-PEST and paxillin Y31/118F or PTP-PEST and paxillin Y31/118F{Delta}LD4. Cells were plated on fibronectin-coated coverslips for 45 minutes, then fixed and processed for indirect immunofluorescence. Cells were stained with rhodamine-phalloidin to visualize the actin cytoskeleton. Bar, 25 µm. (B) Quantification of cell spreading was accomplished by counting round versus spread GFP-positive cells. A minimum of 100 cells were counted per condition in three independent experiments. Results show mean±s.d. **P<0.001, *P<0.05.





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