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Fig. 1. Expression of p75^NGFR, SMA and GFAP in the E14.5 rat forebrain and cranial mesenchyme. Fluorescent antibody staining of E14.5 rat embryo at the telencephalic level. Illustration at upper left orients panels A-G. Red rectangle with asterisk illustrates the cortical region dissected for all experiments. (A) p75^NGFR (green) and smooth muscle -actin (red) show little or no expression in brain but high levels of p75^NGFR in cranial mesenchyme and peripheral ganglia. Arrow indicates choroid plexus. (B-D) Higher magnification, showing (B) cranial mesenchyme, (C) choroid plexus (arrow) and confocal image of choroid plexus epithelium (CPe) and (D-F) mesenchyme. Notice that some CPm cells are p75^NGFR+/SMA⁺, whereas the CPe shows fainter staining for these markers. (G) CPe shows moderate co-expression of p75^NGFR (green) and GFAP (red), whereas the CPm is strongly p75^NGFR+/GFAP⁺. Cranial mesenchyme is stongly p75^NGFR+ but only weakly GFAP⁺. DAPI (blue) identifies all cell nuclei. Bars, 160 µm (A); 40 µm (B-C, E); 15 µm (D).

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