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Fig. 7. FGF2 and BMP2 induce adult rat SVZ stem cells to dorsalized precursors. Adult SVZ stem cells were expanded in FGF2 with noggin prior to passage. (A) RT-PCR time course analysis of Snail1 and Snail2 expression in passage 1, during FGF2 ± BMP2 exposure. GAPDH expression is shown in reverse-transcribed (+) and non-transcribed (–) GAPDH samples as loading controls. (B-E) Comparison of p75^NGFR expression after low-density plating; clones marked after 5 days expansion (116±54 cells/clone) were further expanded with FGF2 ± BMP2. Expression of p75^NGFR is absent in (B) control cultures but prevalent in (C) BMP2 co-treated cells. Percentage of clones containing (D) at least one p75^NGFR+ cell and total percentage of (E) p75^NGFR+ cells during FGF2 expansion without ( ${square}$ ) or with ( ${diamondsuit}$ ) BMP2. Graphs show mean ± s.e.m. (n=2). Bars 20 µm.

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