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Fig. 3. Histograms of average velocity reveal segregation of granule populations upon stimulation. The histograms show the number of granules at different velocities traveled. The inset bar graphs are derived from Table 1 and show the percentage of granules with speeds >0.4 µm/second, with the error bars representing s.e.m. For all panels, a time series of granule movement was first taken under unstimulated condition and then after each treatment. The treatments were sequential, in the order shown in the symbol legend. Images were taken immediately after KCl was added and 2 minutes after glucose was added. *P<0.001 in a paired t-test. (E) The average velocity values were divided into 24 intervals and an average of net displacement (distance between the positions of a granule at the first and the last time points) of all the granules within each interval was plotted against the average velocity. Because various numbers of granules fall into different velocity intervals, each data point represents an average of net displacement from different numbers of granules. (F) Three granules were selected to represent the three types of motion. Data points were fitted to a moving average, linear regression and second degree polynomial curve using Microsoft Excel and labeled as type i, ii, iii, respectively. The inset shows an enlarged view of curves i and ii.
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