spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Levels of transcription and acetylated-histone H3 on HAC or integrated sites. (A) RT-PCR analysis of the transformed cell lines (S026, S013 and K031) was performed as described in Fig. 1D. The schematic map of 7C5-SV/CMV BAC indicates PCR probe sites. The primers used are, from top: ß-actin, bsr, bsr-pA, right junction, left junction, kanR, ßgeo-pA, ßgeo1. (B) ChIP and real-time PCR analysis of alphoid BAC using antibody against acetylated histone H3 and normal IgG (as a control). Recovery rate of immunoprecipitated DNA against the input DNA is shown in the histogram, error bars give the s.e.m. (n=3). As controls, an endogenous promoter region of the CENP-B gene (PCENP-B) and the pericentromeric satellite 2 repeat loci (sat2) were used.





Right arrow Return to article