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Fig. 4. Distribution of CENP-A and heterochromatin-assemblies on HAC and integrated sites analyzed by ChIP. (A) Positions of PCR probes on alphoid BAC DNA are indicated (left arm: L1, L2, L3 and L4, right arm: R1, R2). ChIP and real-time PCR analysis of the transformed cell lines (S026, K031 and original HT1080) were performed with antibodies against CENP-A and triMet H3-K9, and normal IgG. Recovery rate of immunoprecipitated DNA against the input DNA is shown as histogram with s.e.m. (n=2). (B) ChIP analysis with anti-GFP antibody against YFP-HP1{alpha} (blue) or YFP (white) was performed. Relative enrichment was calculated by dividing the immunoprecipitated fraction of each probe by the one of the endogenous promoter of the CENP-B gene (PCENP-B), and is shown in the histogram. Error bars give the s.e.m. (n=2).





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