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Fig. 3. PINCH1fl/fl and PINCH1–/– mouse embryonic fibroblasts (MEFs). (A) Western blot showing the absence of PINCH1 protein in the PINCH1–/– MEFs and of the PINCH2 protein in both PINCH1fl/fl and PINCH1–/– MEFs. To control the PINCH2 assay, a lysate from PINCH2+/+ liver was also blotted. (B-E) Spreading of PINCH1fl/fl (B) and PINCH1–/– (C) cells and of PINCH1–/– cells transduced with EGFP-PINCH1 (D) and EGFP-PINCH2 (E), respectively. Cells were plated on tissue culture dishes for 16 hours in the presence of serum. Bar, 20 µm.





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