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Fig. 4. Immunofluorescent staining of PINCH1fl/fl (A) and PINCH1–/– (B) cells and of PINCH1–/– cells expressing EGFP-PINCH1 (C) or EGFP-PINCH2 (D). Cells were seeded on fibronectin-coated glass coverslips in DMEM without FCS for 4 hours. PINCH1 was either detected by PINCH1 antibody (A,B) or via the EGFP fusion protein (C). PINCH2 was visualized as EGFP-PINCH2 (D). Paxillin was stained with a commercial antibody and filamentous actin by TRITC-conjugated phalloidin. Bar, 20 µm.





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