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Fig. 8. Expression of deletion mutants of PINCH1 and PINCH2 in PINCH1-null cells. (A) Western blots of cell protein extracts. Lane 1: PINCH1fl/fl cells; lane 2: PINCH1^–/– cells; lanes 3-9: PINCH1^–/– cells expressing, EGFP (3), EGFP-PINCH1 (4), EGFP-LIM1/P1 (5), EGFP- ${Delta}$ LIM1/P1 (6), EGFP-PINCH2 (7), EGFP-LIM1/P2 (8), EGFP- ${Delta}$ LIM1/P2 (9). (B) Northern blot of parental fl/fl cells (lane 1) and PINCH1^–/– cells (lane 2). (C) Detection of ILK co-immunoprecipitated with the EGFP-tagged PINCH constructs using an anti-GFP antibody. Lane 1: lysate of PINCH1fl/fl cells; lane 2: negative control co-immunoprecipitation of PINCH1fl/fl cells expressing EGFP; lanes 3-8: PINCH1^–/– cells expressing, EGFP-PINCH1 (3), EGFP-LIM1/P1 (4), EGFP- ${Delta}$ LIM1/P1 (5), EGFP-PINCH2 (6), EGFP-LIM1/P2 (7), EGFP- ${Delta}$ LIM1/P2 (8). (D-G) Localization of the EGFP-tagged constructs LIM1/P1 (D), ${Delta}$ LIM1/P1 (E), LIM1/P2 (F), ${Delta}$ LIM1/P2 (G); FAs were visualized by vinculin staining and arrowheads indicate points of partial co-localization of the constructs with vinculin. Bar, 20 µm.

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