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Fig. 3. Endocytotic vesicle size is regulated in a bimodal manner by Ca²⁺ entry through voltage-dependent channels in INS-1 cells. Experiments were performed with 10 mM glucose and 0 mM Ca²⁺ in the bath, and pipette-Ca²⁺ was varied. (A) The frequency of endocytotic events was unchanged at different pipette-Ca²⁺ concentrations. (B) Inclusion of 2.6 mM Ca²⁺ in the patch-pipette significantly increased the unitary capacitance of endocytotic steps, indicating an increase in vesicle size. This effect was ameliorated by further increasing pipette-Ca²⁺ to 10 mM. (C) With 2.6 mM Ca²⁺ in the patch-pipette, inclusion of the L-type Ca²⁺ channel inhibitor isradipine (israd., 2 µM) or the R-type Ca²⁺ channel inhibitor SNX-482 (100 nM) prevented the Ca²⁺-dependent increase in endocytotic vesicle capacitance. ***, P<0.001.

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