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Fig. 7. Acceleration of membrane fission by Ca2+ in inside-out excised membrane patches from INS-1 cells. Changes in fission pore conductance (Gp) were fit with single exponential decay functions. Bath application of a 100 nM free-Ca2+ buffer or 0.5 mM Ca2+ accelerated the kinetics of membrane fission. (A) Representative Gp traces with 0 mM Ca2+, 100 nM free-Ca2+ or 0.5 mM Ca2+ in the bath. (B) Fission pore time constants ({tau}) were calculated by fitting the Gp traces with single exponential decay functions. Unlike what was seen with endocytotic vesicle size, the effect of Ca2+ on fission kinetics was not bimodal. *, P<0.05; **, P<0.01.





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