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Fig. 8. The Ca²⁺-dependent increase in endocytotic vesicle size, but not the Ca²⁺-dependent acceleration of fission kinetics, is prevented by inhibition of calcineurin. Experiments were performed on inside-out excised membrane patches from INS-1 cells in the presence of a 100 nM free-Ca²⁺ buffer. (A) The frequency at which endocytotic events occurred was not changed by the calcineurin inhibitor deltamethrin (1 µM), but was increased in the presence of exogenous purified calcineurin (0.4 U/ml) and calmodulin (100 U/ml). (B) Antagonism of calcineurin with deltamethrin prevented the Ca²⁺-induced increase in endocytotic vesicle capacitance, whereas exogenous calcineurin/calmodulin had no effect. The control condition in this case represents vesicle capacitance during 100 nM free-Ca²⁺ stimulation. (C) Neither antagonism of calcineurin with deltamethrin nor exogenous calcineurin/calmodulin significantly affected the rate of membrane fission during 100 nM free-Ca²⁺ stimulation. *, P<0.05; ***, P<0.001.

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