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First published online January 26, 2005

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Parasite export route defies convention

Most secreted proteins are exported from cells via the ER, Golgi and secretory vesicles. Some - notably fibroblast growth factor (FGF) and interleukin 1ß - instead avoid the ER and, rather than entering secretory vesicles, use unidentified machinery to cross the plasma membrane (PM). Walter Nickel and co-workers are charting these `unconventional' trafficking routes and have now taken the first step towards identifying the elusive transporters involved (see p. 517). Monitoring unconventional trafficking of the Leishmania lipoprotein HASPB, they have used retroviral mutagenesis to generate CHO cells that cannot export the protein. In the mutants, HASPB accumulates immediately below the PM but cannot cross it - presumably because of a transporter defect. The authors note that initial transport of HASPB to the PM requires acylation of the protein. They therefore conclude that export is a two-step process in which acylation delivers HASPB to the PM and the protein is then translocated across it by transporters that reside there rather than in other membrane systems. Interestingly, Nickel and co-workers find that unconventional trafficking of FGF-2 is unaffected in the mutant CHO cells. This indicates that they must lack a cargo-specific component of the translocation machinery.

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Related articles in JCS:

Direct transport across the plasma membrane of mammalian cells of Leishmania HASPB as revealed by a CHO export mutant

Carolin Stegmayer, Angelika Kehlenbach, Stella Tournaviti, Sabine Wegehingel, Christoph Zehe, Paul Denny, Deborah F. Smith, Blanche Schwappach, and Walter Nickel
JCS 2005 118: 517-527. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?