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Fig. 3. Program of MT-cortex interactions set by Bud6p. Accumulation of Bud6p at the incipient bud directs astral MTs from the old SPB (dark blue) to undergo capture at the new bud site. Following bud emergence, interactions continue with the bud cortex decorated by Bud6p. As the spindle assembles, Bud6p begins to concentrate at the bud neck. This causes the old pole to interact with the bud and bud neck cortex whereas the new pole (light blue) is prevented from interacting with the bud. Once polarity is established, the spindle orientates along the mother-bud axis. Bud6p continues to cue cortical interactions during anaphase and following mitotic exit to redirect the SPBs to contact the division site (not shown but see Segal et al., 2002).





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