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Fig. 4. Separate mechanisms for Bud6p and Kar9p in astral MT capture. (A) Kar9p-mediated MT delivery and Bud6p-dependent capture coexist during pre-anaphase spindle orientation. On average, SPBs are associated with three astral MTs but only one is usually bound to Kar9p at any given time in wild-type cells. A Kar9p-bound MT maintains the SPB orientated towards the bud, whereas other MTs undergo Bud6p-dependent capture at the cell cortex to promote SPB movement coupled to MT growth or shrinkage. In addition, Kar9p might affect dynamicity, which explains the processive transport of Kar9p-bound MTs along actin cables and their persistence within the bud. (B) MT dynamic behaviour in association with Kar9p. A Kar9p-bound MT grows (a) and encounters Myo2p (b) to be transported along an actin cable. Transport towards the bud occurs without changes in MT length (b-d). This mode of delivery can be coupled to SPB movement towards the bud (d). (C) MT dynamic behaviour in association with Bud6p cortical sites. A MT encounters cortical Bud6p (a). This results in SPB movement towards the cortex coupled to MT depolymerization (b). Following spindle assembly, this mode of interaction continues to tether the SPB to the bud neck region.





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