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Fig. 2. Representative series of confocal sections of blastocysts injected at the 4-cell stage with DsRed mRNA, dsPar3 RNA and dominant negative aPKC mRNA. Nuclei fluoresce green, reflecting expression of GFP-tagged histone H2B. The clone formed by the progeny of the injected cells is marked in red. The mean proportions of labelled ICM cells to total labelled cells was 0.34 for DsRed-injected embryos (n=38); 0.53 for dsPar3 RNA-injected embryos (n=16); and 0.71 for dominant negative aPKC-injected embryos (n=25). Bar, 10 µm.





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