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Fig. 6. Recruitment of tight junction proteins following expression of dominant negative aPKC. (A-D) Localization of the tight junction protein ZO1 at the late 8-cell stage in embryos injected with mRNA for dominant negative aPKC and DsRed into one 4-cell-stage blastomere. ZO1 (green and monochrome) localizes to apposing cell surfaces (arrows) between both injected (A,B; injected cells are red and marked with an asterisk) and non-injected (C,D) progeny; 11 embryos were analysed. (E,F) Similar embryo at the blastocyst stage. Occludin (green and monochrome) localizes to the tight junction (arrow) in both non-injected and injected progeny; 9 stained embryos were analysed. The latter are red and marked with an asterisk. Bar, 10 µm.