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Fig. 2. Swimming directions of the wild-type cells and various phototaxis mutant cells 5 seconds after the phototactic light was turned on. (A-D) Polar histograms showing the swimming directions when the light (wild type, 8.8x1016 photons m-2 s-1; lsp1, ptx1 and ida1, 2.2x1018 photons m-2 s-1) was applied in the direction indicated by arrows. Bars show the proportions of cells swimming in the direction binned at 15°. The interval between dotted circles represents 5% of the cells. Data on a total of 420 cells were collected from three experiments. (E,F) Stimulus-response curves for the phototaxis in the wild type (squares) and three mutant cells (lsp1, circles; ptx1, triangles; ida1, crosses). The magnitudes of phototaxis are shown by photo-orientation index calculated from the direction of swimming relative to the direction of light. Dotted line shows the value when the cells exhibit no phototaxis. Each point represents the average and standard deviation from three experiments (140 cells in each experiment).





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